Table 1 The sequence of gRNAs and primers
From: Caspase-7 deficiency in Chinese hamster ovary cells reduces cell proliferation and viability
| Oligoes | Caspase 7 target site | sequences |
|---|---|---|
| Exon 4 | agatggcgtgacgccaataa |
Fw: CACCgAGATGGCGTGACGCCAATAA Rev: AAACTTATTGGCGTCACGCCATCT |
| Exon 4 + PAM | agatggcgtgacgccaataaagg |
Fw: caccgCCTTTATTGGCGTCACGCCATCT Rev: aaacAGATGGCGTGACGCCAATAAAGGc |
| Exon 5 | gatacgctttaggcatgccg |
Fw: CACCGATACGCTTTAGGCATGCCG Rev: AAACCGGCATGCCTAAAGCGTATC |
| Exon 5 + PAM | gatacgctttaggcatgccgagg |
Fw: caccCCTCGGCATGCCTAAAGCGTATC Rev:aaacGATACGCTTTAGGCATGCCGAGG |
| PX-460-1 primers | – |
Fwd: GCCTTTTGCTGGCCTTTTGCTC Rev:CGGGCCATTTACCGTAAGTTATGTAACG |
| Genomic primers | – |
Fwd: CTGAGGAGGACCACAGCAACTC Rev: AACTGTGGAGTAAGCGAAGAGAA |