Fig. 5

Gel image of genomic cleavage detection assay of luteal cells transfected with Cas9 and EGR1 SgRNA using Lipofectamine 2000. Lane 1, 50 bp DNA ladder; Lane 2, negative control sample for EGR1 gene; Lane 3 sample without T7/E1 enzyme, Lane 4: sample, showing parent and both the cleaved bands after addition of T7/E1 enzyme